Viewing the Pangenome

Node Types

segments

Segments = S lines from GFA files (blue). Links = L lines from GFA files (gray).

bubble and chain

A chain of bubbles. Each bubble in the chain is shown in yellow, the chain links are orange.

PangyPlot First displays top-level bubbles. Bubbles can be popped to reveal finer-scale details.

Bubbles in PangyPlot

Iterative bubble popping to the segment level.

Gene Annotations

The gene annotations are provided during setup up PangyPlot. The HPRC live instance uses annotations from GENCODE (any GFF3 file can be used).

Annotations are rendered as outlines around nodes and edges:

gene annotation

Example of rendered gene annotations.

Interactions

Pan/Zoom Mode default
Click and drag to pan the view. Use mouse wheel to zoom in and out.
Bubble Pop Mode ctrl key/cmd key
Click nodes to pop bubbles.
Selection Mode shift key
Click nodes to select. Drag to create a selection rectangle.
Recenter on Subgraph space bar
Press the space bar to recenter the view on the full subgraph.
Recenter on Selection up arrow
Press the up arrow key to recenter the view on the selected nodes.
Anchor on Drag F key
Press the F key to toggle whether a node position is fixed after dragging.

Right-Click Menu

The right-click menu is context-sensitive: the available actions depend on what is under the cursor and whether you have a selection.

Right-click a chain to act on it:

Flip Chain
Reverse the orientation of the chain.
Pop All Bubbles
Expand every bubble along the chain.
Add Custom Annotation
Name and label the chain (or the current selection).

With a set of chains highlighted (Shift-drag to select), act on the selection:

Copy Approx Coordinates
Copy the coordinate range spanned by the selection.
Pop Highlighted
Expand every bubble within the selection.
Export GFA
Export the selected region as a GFA file (detail view).
Export GFA + Layout
Export the selected region as a GFA file along with its layout, in odgi and Bandage formats (detail view).

Export the current view (always available):

Download PNG
Save the current view as a PNG image.
Download SVG
Save the current view as an SVG vector image.